Home > Research

Research


Dr. Radhakrishna S. Pandit
rspandit@unipune.ac.in
+91-20-25601436  ext : 59

In my lab, I am working on following areas: Entomology, Applied Entomology
1 Insect pest Management-.Biological Control of Helicoverpa armigera (Hubner) by using Insect Parasitoids, Pathogens -microbial Insecticides, Insect and their host plants interactions
2 Vector Biology-.Dengue and Filariasis Vector control by using plant extracts .
3.Molecular changes during the Interactions of Entomopathogenic Fungi, bacteria against Helicoverpa armigera
4.Foraging Behavioural and Floral Preference of Honey bees
5.Ecobiology, Behavioral and Biochemical aspects of Insect pests and Parasitoids.
6.Interaction of Vibrio spp with non biting midges (Diptera)

Recent five Publications:

  • Pandit, R. S., Sharbidre A. A. and Jaybhay. Y. S. (2009) Effect of Temperature on Development and Survival of Trichogramma brasiliensis an egg parasitoid of Helicoverpa armigera (Hubner). National J. Life Sciences, 6:169-172.

  • Bagde, U.S. and Pandit, R. S. (2009) Antagonistic Effect of Bacillus thuringiensis subsp. H12 on Pathogens of Tilapia species. Asian Jr. Microbiol. Biotech. Env. Sc., 11:917-922.

  • Sirsath, M.S., Pandit, R. S., and Bagde U.S. (2008) Mechanisam of Action of Ageratum conyzoids plant extract on pathogenic bacteria. National J. Life Sciences, 5:147-152.

  • Sirsath, M.S., Pandit, R. S., and Bagde U.S. (2008) Evaluation of Antimicrobial activity of Ageratum conyzoids plant extract. National Jr. Life Sciences Vol. 5 (1): 7-13

  • Pandit, R. S. (2008) Effect of Temperature on Development and survival of Trichogramma chilonis Ishii, an egg parasitoids of Helicoverpa armigera (Hubner), Bionano Frontier, 1:164-166.

Research Students currently working:

Prof. Deepti D. Deobagkar
dddeo@unipune.ac.in
+91-20-25601436  ext : 33

My laboratory is involved in following areas of research:
Epigenetics and functional genomics: We have been designing new tools and techniques for high throughput genome wide analysis of DNA methylation. This approach enables one to correlate gene expression microarray data to changes in DNA methylation in response to development, differentiation, stress and adaptation. The methylation is assessed in a sequence context independent fashion in a very rapid and reliable manner. It forms a baseline for evaluating the response and generates a methylation landscape. We have investigated the X chromosome inactivation and chromosome function in the context of evolutionary domains, sequence context and microRNA. Role of DNA methylation in radiation damage and its link

to cell ptroliferation, apoptosis and signal transduction is being explored. Role of DNA methylation in Drosophila and Deinococcus radiodurans is also being analysed. A novel methyltransferase assay has been developed for investigating the role of molecular genetic machinery involved in regulation of DNA methylation.
We have designed and developed biosensor and nanotechnology based approaches for rapid, sensitive and specific detection of microbes and ligands.

Selective Recent five Publications:

  • Kelkar A, Deobagkar D. (2010) Methylation profile of genes on the human X chromosome. Epigenetics, 5 [Epub ahead of print]

  • Kelkar, A., Thakur, V., Ramaswamy, R. and Deobagkar Deepti (2009) Characterisation of inactivation domains and evolutionary strata in human X chromosome through Markov segmentation. PLoS One, 4:e7885.

  • Kelkar, A. and Deobagkar Deepti (2009) A novel method to assess the full genome methylation profile using monoclonal antibody combined with the high throughput based microarray approach Epigenetics 4:415-420

  • Ozarkar A.D., Prakash, D.I, Deobagkar D.D. (2007) Prediction of B cell and T cell epitopes of DBL domain in Plasmodium falciparum malaria vaccine candidate Var gene. Protein and Peptide Letters 14: 528-530.

  • Kalele, S.A., Kundu, A. A., Gosavi, S. W., Deobagkar, D. N., Deobagkar, D.D., Kulkarni, S. K.(2006) Rapid Detection of Escherichia coli by Using Antibody-Conjugated Silver Nanoshells. Small, 2:335-338.

Research Students currently working:

Prof. Saroj S. Ghaskadbi
ssg@unipune.ac.in
+91-20-25601436  ext : 24

My laboratory is involved in studying the ability of cells to cope up with oxidative stress using two model systems namely islet cells and liver cells. Our data has shown that compared to liver cells, islet cells are very poor in both their antioxidant defense and their ability to repair oxidative damage to DNA. Treatment of islet cells with antioxidants significantly improves their ability to tolerate oxidative stress. Efforts are being made to understand the mechanism by which these antioxidants work.

Selective Recent five Publications:

  • Jhankar Acharya and Saroj Ghaskadbi (2010) Islets and their antioxidant defense. Islets 2:4-11.

  • Priyanjali Dixit, Mujumdar A.M., Misar A. and Saroj Ghaskadbi (2010) Pre-treatment of Syndrex® protects mice from becoming diabetic after streptozotocin injection. Fitoterapia, 81:403-412.

  • Manisha Modak, Pradeep Parab and Saroj Ghaskadbi (2009) Pancreatic islets are very poor in rectifying oxidative DNA damage. Pancreas, 38:23-29.

  • Jayant Londhe, T.P.A. Devasagayam, L.Yeap Foo and Saroj Ghaskadbi. 2008. Antioxidant activity of some polyphenols of the medicinal plant Phyllanthus amarus. Redox Report, 13:199-207.

  • Priyanjali Dixit, T.P.A. Devasagayam and Saroj Ghaskadbi (2008) Formulated antidiabetic preparation, Syndrex® has a strong antioxidant activity. European Journal of Pharmacology. 581:216-225.

Research Students currently working:

Dr. Bimalendu B. Nath
bbnath@unipune.ac.in
+91-20-25601436  ext : 25

My laboratory is interested in understanding cytogenetic, cellular and whole organismal responses to extreme environmental stress conditions using Chironomus and Drosophila as model organisms. One of the primary research goals of my research team is to understand the cellular and molecular basis of stress tolerance of natural population in contrast to inbred laboratory population. The investigations include hypoxic, andydrobiotic, radiation as well as temperature induced stress responses. Additionally, behavioral responses at the whole organismal level are being explored using multidisciplinary approaches. We believe that through the parallel study of stress-induced adaptive processes an evolutionary insight could be gained about the biology of the ancient extremophilc chironomid midges and its phylogenetically distant dipteran counterpart Drosophila.

Selective five Publications:

  • Datkhile, K. D., Mukhopadhyaya, R., Dongre, T. K. and Nath B. B. (2010) Hsp70 expression in Chironomus ramosus exposed to gamma radiation. International Journal of Radiation Biology [ in press] DOI. 10.3109/09553002.2010.518215.

  • Datkhile, K. D.,Mukhopadhyaya, R., Dongre, T.K., Nath B. B. (2009) Increased level of superoxide dismutase (SOD) activity in larvae of Chironomus ramosus subjected to ionizing radiation. Comparative Biochemistry & Physiology Pt-C 149: 500-506.

  • Nath B. B., Babrekar, A. A., Parthasarathy, B. (2005) Assessment of cell viability in intact glandular tissue using dye-exclusion and colorimetric assays. Cytotechnology 49:59-65.

  • Naik D. G., Babrekar A. A., Nath B. B. (2006) Pheromone-like hydrocarbon in the cuticle of aquatic Chironomus larva. Chemistry & Ecology 22:501-508.

  • Hardikar A.A., Nath B. B. (2001) Chromosomal polymorphism is associated with nematode parasitism in natural population of tropical midge. Chromosoma 110: 58-64

Research Students currently working:

  • Rahul Gaikwad rahul0880@gmail.com (Ph.D. student)

  • Leena Thorat leenathorat@gmail.com (Ph.D. student)

  • Anupama Ronad anupama@hbcse.tifr.res.in (Ph.D. student)

  • Ashok Ghadge ghadgeas008@gmail.com (M.Phil student)

  • Richa Joag richajoag@gmail.com (J.R.F.)
     

Dr. Kalpana Pai
kalpanapai@unipune.ac.in
+91-20-25601436  ext :43

My laboratory is involved in following areas of research:

1. Screening of molecules of diverse origin and herbal Ayurvedic drugs for Immuno-modulatory and anti-leishmanial activity with particular reference to macrophage activation: The effects of some of the herbs and their various preparations on macrophage activation in vitro are being investigated as very little is known about the effect of Ayurvedic drugs on the macrophage activation. Also studies involving evaluation of drug targets in promastigote and amastigote forms of Leishmania are underway

2. Biodiversity and Taxonomic studies with reference to ecology on Freshwater Rotifera, Branchiopoda, Cladocera and Ostracoda from Pune, Maharashtra: The study includes collection and sampling of Planktonic, Epiphytic and Sessile Rotifers from above mentioned sites and immediate transfer to the laboratory. Visits to unexplored sites as Tamhini Forest and Forts are also being undertaken. Till date the studies have revealed several species and new records from the above-mentioned sites.

Selective five Publications:

  • Padhye, S.M., Ghate, H. and Pai K. (2010) New locality record and additional information on habit of Cyclestheria hislopi (Baird, 1859) (Crustacea:Branchiopoda: Cyclestherida) in India. Journal of Threatened Taxa [In Press].

  • Vanjare A. I., Padhye, S.M. and Pai K. (2010) Zooplankton from a polluted river, Mula (India), with record of Brachionus rubens (Ehrenberg, 1838) epizoic on Moina macrocopa (Straus, 1820) Opusc. Zool. Budapest, 41:89-92.

  • Vanjare A. I. and Pai K. (2010) Rotifers from a pond in Pune (India), with the record of Ptygura pedunculata (Edmondson, 1939) (Rotifera: Monogononta) from the Oriental region. Turk. J. Zool., 34:417-419.

  • Tripathi K., Kumar R., Bharti K., Kumar P., Shrivastav R., Sundar S. and Pai K. (2008) Adenosine deaminase activity in sera of patients with visceral leishmaniasis in India. Clinica Chimica Acta, 388:135-138.

  • Kumar, R., Pai, K., Kumar, P., Pandey, H.P. and Sundar, S. (2006) Sero-epidemiological study of kala-azar in a village of Varanasi district, India. Trop Med Int Health, 11:41-8.

  • Sundar ,S., Agrawal, S., Pai, K., Chance, M. and Hommel, M. (2005) Detection of leishmanial antigen in the urine of patients with visceral leishmaniasis by a latex agglutination test. Am J Trop Med Hyg., 73:269-71.

Research Students currently working:

Dr. Sangeeta V. Pandit
drpanditsv@unipune.ac.in
+91-20-25601436  ext : 41

My laboratory is involved in following areas of research:
Lamellidens marginalis is fresh water bivalve which is edible & plays an important role as bio-indicator to detect various environmental fluctuations & aquatic characteristic changes. They also form an important sink for salts, used in various industries such as rayon; paper; cement & fertilizer industries. Size & Season specific variations in Proteins; Lipids, Glycogen, &Ascorbic acid from different tissues of animal are estimated. . Estimation of accumulation of non bio-degradable pesticides in different organs of body is intended. Since molluscs constitute important organism in food chain such studies can be extended to other organisms belonging to different taxonomic groups.

Currently I am working on following projects:
1. Histopathological studies on different organs of fresh water bivalve exposed to different pesticides.
2 .Modelling spatial patterns of fresh water reservoirs with respect to mollusks, planktons and fishes using physico-chemical parameters of water and remote sensing techniques.

Recent five Publications:

  • Vedpathak, A. N. and Pandit S.V(2009) Ichthyofauna of Bhandardara Reservoir in relation to Bird Diversity, Flora and Fauna, 15:91-94.

  • Vedpathak, A. N. and Pandit S.V.(2008) Size &season specific variations in the oxygen consumption & O:N ratio of Fresh water bivalve Lamellidens marginalis from Godavari river near Aurangabad. Uttarpradesh Jr. of Zoology 28:231-238.

  • Pandit, S. V. and Vaidya V.V. (2007) Studies on Zooplankton diversity of Pravara river near Sangamner Dist Ahmednagar. Jr. of aquatic Biology, 22:33-38.

  • Pandit, S. V. and Vedpathak, A N. (2006) Size &season specific variations in the protein contents of Fresh water bivalve Lamellidens marginalis from Godavari river near Aurangabad. Jr. of aquatic Biology, 21:36-38.

Research Students currently working:

Dr. Varsha W. Wankhade
varsha3w@unipune.ac.in
+91-20-25601436  ext : 60

My laboratory is involved in following areas of research:
Sickle cell anemia: Sickle-cell anemia (SS) is a genetic disorder caused by sickle hemoglobin (Hgb S or Hb S). The disease is chronic and lifelong. Sickle cell anemia is an inherited blood disorder characterized primarily by chronic anemia and periodic episodes of pain.

Individuals are most often well, but their lives are punctuated by periodic painful attacks. Life-expectancy is shortened, but contemporary survival data is lacking. Earlier studies indicate that sufferers could live to an average of 40 to 50 years, with the average age for males being 42 and the average age for females being 48. The mutated allele has incomplete dominance. The high prevalence of sickle cell disease among the Indian population especially those of central and South India is largely undetected or underdetected because of lack of awareness and testing facilities among the middle and lower income groups belonging to these regions.

We are involved in the screening of population for SSA , study of the pathophysiological status of sickle cell anemic patients and we will also deal with the genetic aspect of SSA in future .

Biodiversity: our lab is involved in the study of biodiversity of some invertebrates especially insects in different regions of Maharashtra. We also are studying the biodiversity of fresh water reservoirs in Maharashtra.

Physiology and toxicology: Our lab is also involved in the study effect of toxicity of some drugs on physiology and biochemistry of the animals like mice. I am also working on on stress physiology. The laboratory welcomes collaborations with patients, clinicians, and scientists, research and teaching institutes and actively seeks patients and families with SSA who may be interested in participating in this research. Students in my lab laboratory are exposed to various techniques needed for the project work.

Recent five Publications:

  • Wankhade, Varsha and Kulkarni K. M. (2010 ) Inhibition of acetylcholinesterase in mice liver by dieldrin. Environment and Ecology, 28: 1795-97.

  • Varsha Wankhade and K.M.Kulkarni. (2010) Effect of dieldrin toxicity on brain AchE activity of mice. Environment and Ecology 28:1179-1182.

  • Wankhade, Varsha, Kulkarni K.M. and Malu A.R. (2009) Effect of malathion on plasma AchE activity of mice. Environment and Ecology, 27: 854-856.

  • Wankhade, Varsha, Malu A.R. and Pawar S. S.(2009) Effect of malathion on liver ache activity of mice. Biology and edicin, 1: 122-126.

  • Wankhade, Varsha, Kulkarni K.M. and Malu A.R. (2008) Effect of malathion on brain ache activity of mice. Environment and Ecology, 26:494-496.

Research Students currently working:

 

Dr. Shobha Y. Bhargava
shobha@unipune.ac.in
+91-20-25601436  ext : 39

My laboratory is involved in following areas of research:

1. Neuroendocrinology: Olfactory system is the sensory system used for olfaction. Olfactory systems are able to detect and distinguish thousands of molecules in the environment.Olfactory sensory neuron is the primary transduction unit for olfaction in the olfactory system. Endowed with hundreds of different receptor molecules to bind odorants and transfer the chemical signals into chemosensory signal. They convey this information in the glomerula of olfactory bulb to the higher centers of brain. My lab is trying to; understand the role of peptides in the olfactory signaling of anuran amphibians.Here, we show that small peptides serves as ligands for major histocompatibility complex molecules and function as sensory stimuli. Using a combination of immunohistochemical techniques and enzyme assays.

2. Developmental Biology: Early development of vertebrates comprises of several milestones and one of the most important amongst them is neurogenesis. In the avian embryo, morphogenetic cell death occurs during the formation of neural tube, lens vesicle and the heart. Immediately after birth, excess neurons in specific regions of the brain die in order to ensure correct region-specific patterning. Our lab uses chick embryo as a model system for studying the patterning of neural tube during early development of chick embryo. We are also studying the modulation of growth factors exposed to ionizing radiation during early development of nervous system.

Selective Recent five Publications:

  • Anupama Anand Mathi , Tekchand C. Gaupale , Corinne Dupuy , Nishikant Subhedar, Shobha Bhargava (2010) Expression pattern of iodotyrosine dehalogenase 1 (DEHAL1) during the ontogeny of chick. International Journal of Developmental Biology (in press).
  • Sudha Ponnusamy, Remya Ravindran, Smita Zinjarde, Shobha Bhargava, and Ameeta Ravi Kumar, (2010) Evaluation of Traditional Indian Antidiabetic Medicinal Plants for Human Pancreatic Amylase Inhibitory Effect In Vitro. Evid Based Complement Alternat Med. vol. 2011, 10 pages, doi:10.1155/2011/515647.
  • Menakshi Bhat, Sandeepkumar K. Kothiwale, Amruta R. Tirmale, Shobha Y. Bhargava and Bimba N. Joshi (2009) Antidiabetic Properties of Azardiracta indica and Bougainvillea spectabilis: In Vivo Studies in Murine Diabetes Model. Evid Based Complement Alternat Med. eCAM 2009:1-8.
  • Gaupale T.C., Mathi A.A., Ravikumar A., Bhargava S.Y. (2009) Localization and enzyme activity of iodotyrosine dehalogenase 1 during metamorphosis of frog Microhyla ornata. Ann N Y Acad Sci., 1163:402-6.
  • Bhargava S. (2007) Role of nerve growth factor and its receptor in the morphogenesis of neural tube in early chick embryo. Gen Comp Endocrinol. 153:141-6.

Research Students currently working:

Dr. Narahari P. Gramapurohit
naraharipg@unipune.ac.in
 
+91-20-25601436  ext : 47

1. Sex determination studies: My laboratory is involved in understanding the factors (endogenous and exogenous) that control sex determination and gonadal differentiation, development and sexual maturation in Indian amphibians particularly anurans. We have shown that anurans exhibit unique pattern of gonadal differentiation they are either differentiated, undifferentiated or semi-differentiated. We have also shown that gonadal differentiation precedes steroidogenesis in one semi-differentiated frog and that exogenous sex steroids influence gonadal differentiation.

2. Behavioural ecology studies: I am also interested in understanding the factors that control/regulate larval growth, development and metamorphosis of Indian anurans (Kin recognition, prey-predator interaction etc are of particular interest).

Recent five Publications:

  • Gramapurohit, N. P. 2009. Catch-up growth during juvenile life can compensate for small metamorphic size in Euphlyctis cyanophlyctis. Curr. Sci. 97:1243 – 1247.

  • Gramapurohit, N. P., Shanbhag, B. A. and Saidapur, S. K. 2008. Kinship affects growth and metmaoprphic traits of Bufo scaber in context dependent manner. J. Herpetol., 42:39-45.

  • Gramapurohit, N. P., Veeranagoudar, D. K. Mulkigoudra, S. V. Shanbhag, B. A. and Saidapur, S. K. 2006. Kin recognition in Bufo scaber tadpoles: Ontogenetic changes and mechanism. J. Ethology., 24:267-274.

  • Gramapurohit, N. P., Shanbhag, B. A. and Saidapur, S. K. (2000) Pattern of sex differentiation, Development and Onset of steroidogenesis in the frog, Rana curtipes. Gen. Comp. Endocrinol., 119: 256-264.

  • Saidapur, S. K., Gramapurohit, N. P. and Shanbhag, B. A. (2001) Effect of sex steroids on gonadal differentiation and sex reversal in Rana curtipes. Gen. Comp. Endocrinol., 124:115-123.

Research Students currently working:

Dr. Richa Ashma
richaashma@unipune.ac.in
+91-20-25601436  ext : 40

My laboratory uses genome wide approach to identify genetic basis of osteoporosis and osteopenia and a wide range of techniques in human genetics to elucidate the pathogenesis of diseases. Techniques include family genetic linkage as well as population genetics analysis, GWAS, and transcriptional genomics. The laboratory welcomes collaborations with patients, clinicians, and scientists, and actively seeks patients and families with osteoporosis who may be interested in participating in this research. Trainees in the laboratory are exposed to a breadth of techniques in statistical genetics, molecular genetics, and molecular biology.

Recent five Publications:

  • Trivedi, R. Sanghamitra Sahoo, Anamika Singh, G. Hima Bindu, Jheelam Banerjee, Manuj Tandon, Sonali Gaikwad, Revathi Rajkumar, T. Sitalaximi, Richa Ashma, G.B.N. Chainy and V. K. Kashyap. (2008) Genetic Imprints of Pleistocene Origin of Indian Population: A comprehensive Phylogeographic Sketch of Indian Y- Chromosomes. International Journal of Human Genetics, 8: 97-118

  • Gaikwad, Sonali Richa Ashma, Nirbhay Kumar, Rajni Trivedi and V K Kashyap. (2005) Host microsatellites in malaria predisposition? Malaria Journal . 4: 50

  • Kashyap, V. K. Richa Ashma, Sonali Gaikwad, B. N. Sarkar and R. Trivedi (2004) Deciphering diversity in populations of various linguistic and ethnic affiliations of different geographical regions of India: Analysis based on 15 mirosatellite markers. Journal of Genetics, 83: 49-63.

  • Kashyap, V. K. and Richa Ashma (2003) Microsatellite diversity in HbS carrier and normal individuals of tribal groups of Malaria infested regions. Special issue, International Journal of Human Genetics, 3: 197-203

  • Richa Ashma and Kashyap V. K. (2003) Genetic profile based upon 15 microsatellites of four caste groups of Eastern Indian state, Bihar. Annals of Human Biology, 38: 570-578.

Research Students currently working:

 

Mrs. Archana A. Sharbidre
aasharbidre@unipune.ac.in
+91-20-25601436  ext : 50

My laboratory is working on following objectives:
i. Estimation of acute toxicity of various organophosphorous insecticides and evaluation of the usefulness of antioxidant parameters in the freshwater fish, Poecilia reticulata, as biomarkers of exposure to pollutants and to study their potential interest in predicting toxicity. Behavioural changes, genotoxic effects and haematological alterations are also investigated.
ii. Use of Musca domestica as an alternative insect model for the study of oxidative stress using various chemicals.
iii. Effect of plant extracts on larvicidal, adulticidal, repellent activities as well as changes in various biochemical aspects of Musca domestica.

Recent Publications:

  • Pandit, R. S., Sharbidre A.A. and Jaybhay. Y. S. (2009) Effect of Temperature on Development and Survival of Trichogramma brasiliensis an egg parasitoid of Helicoverpa armigera (Hubner). National J. Life Sciences, 6:169-172.

  • Kulkarni A.S., Sharbidre A. A., Tendulkar M.V. (2007) Length weight relationship of Istiblennius dussumieri (Val) Ratnagiri Sea coast, Peth Killa region, Ratnagiri. Journal of Aquatic Biology, 22:112-114.
     

Dr. Kedar C. Ahire
kedar_ahire@unipune.ac.in
+91-20-25601436 ext : 81

The work in my laboratory involves use bacterial systems for the degradation of xenobiotic compounds. The knowledge of key pathways involved in biodegradation can be gained through the genomic, metagenomic, proteomic, bioinformatics and other high-throughput analyses of environmentally relevant microorganisms. Also the bioactive compounds from the bacterial and fungal systems are being explored.

The other area of my research is nanobiotechnology, which is a rapidly advancing area which uses the principles of biology for nano/microfabrication. The novel synthetic approaches for magnetic nanoparticles and their applications in biomedicine and sensor applications are being studied.

Recent Publications:

  • Ahire KC, Kulkarni GJ, Shouche YS, Kapadnis BP and Deopurkar RL (2012) Biodegradation of tributyl phosphate by novel bacteria isolated from enrichment cultures. Biodegradation 23:165–176.

  • Ahire KC, Arora MS and Mukherjee SN (2008) Development and application of a method for analysis of lufenuron in wheat flour by gas chromatography-mass spectrometry and confirmation of bio-efficacy against Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). J. Chromatog. B. 861:16–21.

  • Rautaray D, Bansal V, Bharade A, Ahire K, Sanyal A, Ahmed A and Sastry M (2005) Fungus mediated biosynthesis of silica and titania nanoparticles. J. Mat. Chem. 15:2583-2589.
     

 


Copyright ©  Savitribai Phule Pune University. All Rights Reserved.