Saroj Ghaskadbi

With interest in DNA damage and repair, initially we worked with islet cells of mouse and human pancreas and showed that they have very poor ability to repair oxidatively damaged DNA compared to liver cells. We developed monoclonal antibody against 8-OH-dG to quantitate the damage in the DNAin collaboration with National Center for Cell Science, Pune.

Later on, in collaboration with Dr. Surendra Ghaskadbi’s lab at Agharkar Research Institute, Pune, , we have been working on DNA repair systems in Hydra, a cnidarian. Hydra is a simple diploblastic animal that has been around for over 500million years. It exhibits lack of organismal ageing and a spectacular ability to regenerate. Very little is known about DNA repair in hydra. We have identified a few genes in nucleotide excision repair, base excision repair and are currently characterising them.

We are interested in understanding how cells cope up with oxidative stress. We use different cell systems including islet cells, liver cells, muscle cells and adipose cells in this work. Islet cells are insulin producing cells while liver, muscle and adipose cells are insulin responsive cells.We have clearly demonstrated that due to low antioxidant defence, islet cells succumb very easily to oxidative stress leading to impairment of their function of insulin secretion compared to the more robust liver cells. We also showed that treatment with strong antioxidants helps islet cells cope up better with oxidative stress both in vitro and in vivo. We are now trying to understand involvement of oxidative stress in development of insulin resistance in liver, muscle and adipose cells which are insulin responsive.

We have recently studied comprehensive oxidative stress profile of newly diagnosed diabetic patients and checked whether this profile improves on controlling hyperglycemia. Our results clearly demonstrate that reduction in glycemic stress in diabetic patients alleviates oxidative stress, improves beta cell function and reduces insulin resistance. Systematic monitoring of oxidative stress with glucose could identify an individual’s recovery trajectory.  In collaboration with Dr. Pranay Goel, Indian Institute of Science Education and Research, Pune, we developed a mathematical model which showed that recovery is marked with a quantal response. From the model we could predict amount of maximal glutathione at low stress, glucose threshold for half-maximal glutathione and  rate at which recovery progresses. We did find significant differences among individuals in their response to glucose control. We believe that this method can help in personalisation of effective target of glucose control in anti-diabetic therapy.

• Dr. Bhonde Ramesh, National Center for Cell Science, Pune
• Dr. Devasagayam T.P.A, BhabhaAtomic Research Center, Mumbai
• Dr. Ghaskadbi Surendra, Agharkar Research Institute, Pune
• Dr. Goel Pranay, Indian Institute of Science Education and Research Pune, Pune
• Dr. Parab Pradeep, National Center for Cell Science, Pune
• Dr. Yajnik C.S., Diabetic Unit, KEM Hospital, Pune

Based on work related to Diabetes:

1. Amlan Barua, Jhankar Acharya, Saroj Ghaskadbi, Pranay goel, 2014, The relationship between fasting plasma glucose and HbA1c during intensive periods of glucose control in antidiabetic therapy. Journal of Theoretical Biology, 363, 158-163.
2. Rashmi Kulkarni, Jhnakar acharya, Saroj Ghaskadbi, Pranay Goel. 2014, Thresholds of oxidative stress in newly diagnosed diabetic patients on intensive glucose therapy. PLOS one, 9, issue 6, e100897.
3. Rashmi Kulkarni, Jhnakar acharya, Saroj Ghaskadbi, Pranay Goel . 2014, Oxidative stress as a covariate of recovery in diabetes therapy. Frontiers in Endocrinology, 5, Article 89, 1-3.
4. Jhankar D Acharya, Amol J Pande, Suyog M Joshi, Chittaranjan S Yajnik , Saroj S. Ghaskadbi 2014 Treatment of hyperglycemia in newly diagnosed diabetic patients is associated with a reduction in oxidative stress and improvement in β-cell function. Diabetes Metabolism and Reviews. 30, 590-598.
5. Manisha A. Modak , P. B. Parab, Saroj S. Ghaskadbi 2014 Manisha A. Modak , P. B. Parab, Saroj S. Ghaskadbi 2014 Tissue specific oxidative stress profile in relation to glycemic regulation in mice. Diabetes Metabolism and Reviews 30: 31-41.
6. Manisha Modak, Pradeep Parab, Saroj Ghaskadbi. 2011, Control of hyperglycaemia significantly improves oxidative stress profile of pancreatic islets, Islets3:5, 234-240
7. Prajkta V. Belsare, Milind G. Watve, Saroj S. Ghaskadbi, Dattatray S. Bhat, Chittaranjan S. Yajnik, Maithili Jog, 2010, Metabolic syndrome: aggression control mechanisms gone out of control. Medical Hypothesis. 74, 578-589.
8. Anuja Joshi, Sumedha Kondekar, Prajakta Belsare, Saroj Ghaskadbi, Milind Watve, Maithili Jog. 2010. People with Metabolic Syndrome Disorders Give Lower Offers in Ultimatum Game. Psychology, 2010, 1, 128-133.
9. Jhankar Acharya, Saroj Ghaskadbi. 2010. Islets and their antioxidant defense. Islets. 2, 4-11. Manisha Modak, Pradeep Parab, Saroj Ghaskadbi. 2009. Pancreatic islets are very poor in rectifying oxidative DNA damage. Pancreas. 38, (1), 23-29.
10. Priyanjali Dixit, T.P.A. Devasagayam, Saroj Ghaskadbi. Formulated antidiabetic preparation, Syndrex® has a strong antioxidant activity. 2008 European Journal of Pharmacology. 581, 216-225.
11. R Shukla, S Padhye, M Modak, S.S Ghaskadbi, R.R Bhonde. 2007. Bis(quercetinato) oxovanadium IV Reverses Metabolic Changes in Streptozotocin-Induced Diabetic Mice. Rev Diabet Stud. 4:33-43
12. Manisha Modak, Priyanjali Dixit, Jayant Londhe, Saroj Ghaskadbi, T.P.A. Devasagayam. 2007 Indian herbs and herbal drugs used for the treatment of diabetes, Journal Clinical Biochemistry and Nutrition, 40, 163-173.
13. Manisha Modak, Savita Datar, Ramesh Bhonde, Saroj Ghaskadbi. 2007 Differential susceptibility of chick and mouse islets to streptozotocin and its co-relation with islet antioxidant status. J. of Comparative Physiology, 177, 247-257.
14. Priyanjali Dixit, Jayant Londhe, Saroj Ghaskadbi and T.P.A. Devasagayam. 2006 Antidiabetic and related beneficial properties of Indian medicinal plants. In, Herbal Drugs A Twenty First Century Perspective, Eds. R.K. Sharma and R. Arora, Jaypee Brothers Medical publishers, 376-386.
15. Priyanjali Dixit, Saroj Ghaskadbi, Hari Mohan and T.P.A. Devasagayam. 2005 Antioxidant properties of germinated fenugreek seeds, Phytotherapy Research, 19 (11), 977-983.
16. Devasagayam T.P. A., Tilak J.C., Boloor K.K., Sane K., Saroj Ghaskadbi, Lele R.D. 2004 Free radicals and antioxidants in human health: current status and future prospects. Journal of The Association of Physicians of India, 52794-804.
17. Devasagayam T.P. A., Tilak J.C., Harimohan, Sane K., Saroj Ghaskadbi. 2003 Free radical damage as a possible cause of disease development and its prevention by some natural products, ISRAPS Bulletin, 13, 25-30
18. Saroj Ghaskadbi. 2003 Free radicals and diabetes, ISRAP bulletin, 13, 16-19.

Based on work related to oxidative stress and antioxidants:

19. Niranjan A. Pant, Dharmendra K. Maurya, Saroj S. Ghaskadbi, Santosh K. Sandur 2015 Troxorutin, a plant flavonoids, protects cells against oxidative stress induced cell death through radical scavenging mechanism. Food Chemistry, 194, 32-45.
20. Anita Thakur, Md. Jahangir Alam, Ajayakumar MR, Saroj Ghaskadbi, Manish Sharma, Shyamal K. Goswami. 2015 Norepinephrine induced apoptotic and hypertrophic responses in H9c2 cardiac myoblasts are characterized by different repertoire of reactive oxygen species generation. Redox Biology, 5, 243-252.
21. Kailas D. Datkhile, Pallavi S. Gaikwad, Saroj S. Ghaskadbi, Rita Mukhopadhyaya, Bimalendu B. Nath. 2015 Chironomus ramosus larvae exhibit DNA damage control in response to gamma radiation. Int. J. Rad. Biol. (doi: 10.3109/09553002.2015.1062572)
22. N. A. Panat, B. Amrute, Shateesh B., S. K.Haram, G.K.Sharma, Saroj Ghaskadbi. 2015. Antioxidant profiling of C3 quercetin glycosides: Quercitrin, Quercetin 3-β-D-glucoside and Quercetin 3-O-(6''-O-malonyl)-β-D-glucoside in cell free environment. 201 Free Radicals and Antioxidants FRA Vol 5 Issue 2, 90-100.
23. Swati Khole, SuchandraChatterjee, Prasad Variyar , ArunSharma, T.P.A.Devasagayam, Saroj Ghaskadbi. 2014 Bioactive constituents of germinated fenugreek seeds with strong antioxidant potential. Journal of Functional Foods. 6, 270-279.
24. Sangeta Sinha, Saroj Ghaskadbi 2013 . Thearubigins rich black tea fraction reveals strong antioxidant activity. Int. J of Green Pharmacy. 7, Issue 4, 336-344.
25. Jhankar Acharya, Saroj Ghaskadbi 2013 Protective effect of Pterostilbene against free radical mediated oxidative damage. BMC Complementary and Alternative Medicine 2013, 13:238
26. Jayant S. Londhe, Thomas P.A. Devasagayam, L. Yeap Foo, Padma Shastry and Saroj S. Ghaskadbi, 2012, Geraniin and amariin, ellagitannins from Phyllanthus amarus protect liver cells against ethanol induced cytotoxicity. Fitoterapia. 82, 1562-68.
27. Tekchand Gaupale , Jayant Londhe, Saroj Ghaskadbi, N. K. Subhedar and Shobha Bhargava . 2012. Immunohistochemical localization and biochemical changes in catalase and superoxide dismutase during metamorphosis in the olfactory system of frog Microhyla ornate. Neuroscience Research, 72, 140-147.
28. Sangeeta sinha, Jyoti Bhat, Manoj Joshi, Vilas Sinkar, Saroj Ghaskadbi. 2011 Hepatoprotective activity of Picrorhiza kurroa Royle Ex, Benth extract against alcohol cytotoxicity in mouse liver slice culture. Int. J of Green Pharmacy, 5(3), 244-253.
29. Mahnaz Mohamadi, Swati Khole, T.P.A. Devasagayam, Saroj Ghaskadbi. 2010. Genotoxic assessment of Pterocarpus marsupium extract. J. of Complementary and Integrative medicine, 7, issue 1, 1-11.
30. Priyanjali Dixit, Mujumdar A.M., Misar A., Saroj Ghaskadbi. 2010. Pre-treatment of Syndrex ® protects mice from becoming diabetic after streptozotocin injection. Fitoterapia. 81, pp.403-412.
31. Mahnaz Mohamadi, Swati Khole, T.P.A. Devasagayam, S aroj Ghaskadbi, 2009. Pterocarpus marsupium extract reveals strong antioxidant activity. Drug Development Therapeutics, 3(4), 151-161
32. Jayant Londhe, T.P.A. Devasagayam, L.Yeap Foo, Saroj Ghaskadbi. 2009. Radioprotective properties of polyphenols from Phyllanthus amarus Linn. J. Radiation Research, 50, 303-309
33. Jayant Londhe, T.P.A. Devasagayam, L.Yeap Foo, Saroj Ghaskadbi. 2008. Antioxidant activity of some polyphenols of the medicinal plant Phyllanthus amarus. Redox Report 13, (5), 199-207.
34. Sinha S., Sane K., Bhargav S., Devasagayam T.P.A., Saroj Ghaskadbi. 2006 Bark and fruit extracts of Gmellina arborea protect liver cells from oxidative stress in vitro. Pharmaceutical Biology. 44 (4), 237-243.
35. Naik R.S., A.M. Mujumdar, Saroj Ghaskadbi. 2004 Protection of liver cells from ethanol cytotoxicity by curcumin in liver slices in culture in vitro. J. Ethnopharmacology, 2004, 95, 31-37.

Based on work related to Hydra:

36. Apurva Barve, Saroj Ghaskadbi and Surendra Ghaskadbi, 2013. Structural and sequence similarity of hydra xeroderma pigmentosun A protein to human homolog suggest early evolution and conservation, Biomed Research International, Vol. 2013, Article ID 854745.
37. Apurva Barve, Saroj Ghaskadbi, Surendra Ghaskadbi 2013 Conservation of the Nucleotide Excision Repair Pathway. Characterization of Hydra Xeroderma Pigmentosum Group F Homolog PLOS One 8, issue 4, e61062.
38. Vishal Kadu, Saroj Ghaskadbi, Surendra Ghaskadbi, 2012. Induction of secondary axis in hydra revisited:New insights into pattern formation. IJMCM, 1, No 1, 1-10
39. Saroj Ghaskadbi, Leena Shetye, Shashi Chiplonkar and Surendra Ghaskadbi. 2005 Ultraviolet irradiation initiates ectopic foot formation in regenerating hydra and promotes budding. J. Biosciences, 30, 177-182
40. Chougale B., Ghaskadbi S.S., Patwardhan V. and Ghaskadbi S. 2006 Cytochalasin B and taxol modulate cell surface ultrastructure in hydra. Current science, 90, 568-574.

Others:

41. Chaitali Botre, Arjun Shahu, Neeraj Adkar, Yogesh Shouche, Saroj Ghaskadbi, Richa Ashma. 2015. Superoxide dismutase 2 polymorphisms and osteoporosis in Asian Indians: A genetic association analysis. Cellular & Molecular Biology Letters. In press
42. Gaikwad SS, Ghate HV, Ghaskadbi SS, Patole MS, Shouche YS. 2012. DNA barcoding of nymphalid butterflies (Nymphalidae: Lepidoptera) from Western Ghats of India. Molecular Biology Reports 39:2375-2383.
43. Saroj Ghaskadbi and S.P. Modak. 1996 Structural alterations induced in supercoiled SV 40 DNA by Methyl methane sulphonate, in Proceedings of a symposium on Environmental mutagenesis and Carcinogenesis, vol. II, 4-9.
44. Saroj S. Ghaskadbi, V. Apate and S.P. Modak. 1995 Fast inducible repair of microinjected UV- irradiated SV40 DNA in monkey kidney cells. Cell Mol. Biol. Rep. 41, 67-72.
45. Saroj S. Ghaskadbi, S. Bharathi, S.P. Modak. 1995 Inhibition of cleavage by restriction endonuclease due to the modifications induced in SV40 DNA by Methyl methane sulphonate. Cell Mol. Biol. Rep. 41, 59-66.
46. A. Rama, S.J. Rajadhyaksha, A. Therwat, N.N. Godbole and S.P. Modak. 1984 Production of mosquito gene library, Current Science, 53, 995-999.

1. Fundamentals of Molecular Biology, (2009) Jayant K. Pal and Saroj S. Ghaskadbi, Oxford University Press

1. JhankarAcharya andSaroj Ghaskadbi. Free radicals and islet function. 2014. InSystems Biology of Free Radicals and Anti-Oxidants, Ed. IssyLaher, Springer Verlag Publishers. 3339-3360.
2. Khole S, Londhe J, Ghaskadbi SS, Devasagayam TPA. 2011. Beneficial effects of Indian Herbs for the treatment of Diabetes. In: Phytochemicals and human health , Ed: Farooqui A and Farooqui T. Nova Science Publishers. Chapter : XII, 315-343.
3. Dixit P, Londhe J, Ghaskadbi SS, Devasagayam TPA. 2006. Anti-diabetic and related beneficial properties of Indian medicinal plants. In: Herbal drugs A twenty frist Century Perspective , Eds. R.K. Sharma and R. Arora, Jaypee Brothers medical publishers. 376-386.